Reconstitution of monoterpene indole alkaloid biosynthesis in genome engineered Nicotiana benthamiana.

ABSTRACT: Monoterpene indole alkaloids (MIAs) are a diverse class of plant natural products that include a number of medicinally important compounds. We set out to reconstitute the pathway for strictosidine, a key intermediate of all MIAs, from central metabolism in Nicotiana benthamiana. A disadvantage of this host is that its rich background metabolism results in the derivatization of some heterologously produced molecules. Here we use transcriptomic analysis to identify glycosyltransferases that are upregulated in response to biosynthetic intermediates and produce plant lines with targeted mutations in the genes encoding them. Expression of the early MIA pathway in these lines produces a more favorable product profile. Strictosidine biosynthesis was successfully reconstituted, with the best yield

SUBMITTER: Dudley QM 

PROVIDER: S-EPMC9464250 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature