RBM15 condensates modulate m⁶A modification of STYK1 to promote tumorigenesis.

ABSTRACT: RBM15 expression is recurrently upregulated in several types of malignant tissues, and its high expression level is typically associated with poor prognosis. However, whether and how RBM15 is involved in the tumor progression remains unclear. In this study, we found that overexpressing RBM15 in NIH3T3 cells was able to enhance proliferation rate in vitro and induced subcutaneous tumor formation in vivo. Moreover, we imaged the subcellular localization of RBM15 with our home-built structured illumination super-resolution microscopy, and revealed that RBM15 formed substantial condensates dispersed in the nucleus, undergoing dynamic fusion and fission activities. These condensates were partially colocalized with m⁶A-modified transcripts in the nucleus. In addition, we

SUBMITTER: Jiang A 

PROVIDER: S-EPMC9464649 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature