IGF1R signalling is a guardian of self-tolerance restricting autoantibody production.

ABSTRACT:

Objective

Insulin-like growth factor 1 receptor (IGF1R) acts at the crossroad between immunity and cancer, being an attractive therapeutic target in these areas. IGF1R is broadly expressed by antigen-presenting cells (APC). Using mice immunised with the methylated albumin from bovine serum (BSA-immunised mice) and human CD14⁺ APCs, we investigated the role that IGF1R plays during adaptive immune responses.

Methods

The mBSA-immunised mice were treated with synthetic inhibitor NT157 or short hairpin RNA to inhibit IGF1R signalling, and spleens were analysed by immunohistology and flow cytometry. The levels of autoantibody and cytokine production were measured by microarray or conventional ELISA. The transcriptional profile of CD14⁺ cells from blood of 55

SUBMITTER: Erlandsson MC 

PROVIDER: S-EPMC9464816 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature