Target-enrichment sequencing yields valuable genomic data for challenging-to-culture bacteria of public health importance.

ABSTRACT: Genomic data contribute invaluable information to the epidemiological investigation of pathogens of public health importance. However, whole-genome sequencing (WGS) of bacteria typically relies on culture, which represents a major hurdle for generating such data for a wide range of species for which culture is challenging. In this study, we assessed the use of culture-free target-enrichment sequencing as a method for generating genomic data for two bacterial species: (1) Bacillus anthracis, which causes anthrax in both people and animals and whose culture requires high-level containment facilities; and (2) Mycoplasma amphoriforme, a fastidious emerging human respiratory pathogen. We obtained high-quality genomic data for both species directly from clinical samples, with suffi

SUBMITTER: Dennis TPW 

PROVIDER: S-EPMC9465068 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature