The phenotype of the gut region is more stably retained than developmental stage in piglet intestinal organoids.

ABSTRACT: Intestinal organoids are innovative in vitro tools to study the digestive epithelium. The objective of this study was to generate jejunum and colon organoids from suckling and weaned piglets in order to determine the extent to which organoids retain a location-specific and a developmental stage-specific phenotype. Organoids were studied at three time points by gene expression profiling for comparison with the transcriptomic patterns observed in crypts in vivo. In addition, the gut microbiota and the metabolome were analyzed to characterize the luminal environment of epithelial cells at the origin of organoids. The location-specific expression of 60 genes differentially expressed between jejunum and colon crypts from suckling piglets was partially retained (48%) in the derived

SUBMITTER: Mussard E 

PROVIDER: S-EPMC9465596 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature