Investigating brain uptake of a non-targeting monoclonal antibody after intravenous and intracerebroventricular administration.

ABSTRACT: Monoclonal antibodies play an important role in the treatment of various diseases. However, the development of these drugs against neurological disorders where the drug target is located in the brain is challenging and requires a good understanding of the local drug concentration in the brain. In this original research, we investigated the systemic and local pharmacokinetics in the brain of healthy rats after either intravenous (IV) or intracerebroventricular (ICV) administration of EGFRvIII-T-Cell bispecific (TCB), a bispecific monoclonal antibody. We established an experimental protocol that allows serial sampling in serum, cerebrospinal fluid (CSF) and interstitial fluid (ISF) of the prefrontal cortex in freely moving rats. For detection of drug concentration in ISF, a push-pull microdi

SUBMITTER: Van De Vyver AJ 

PROVIDER: S-EPMC9465605 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature