Gene expression profile of human colorectal cancer identified NKTR as a biomarker for liver metastasis.

ABSTRACT:

Objective

Liver metastasis is one of the prognostic factors of colorectal cancer (CRC). The aim of this study is to identify biomarkers that facilitate easier detection of liver metastasis.

Methods

Significance Analysis of Microarrays (SAM) and Array Data Analyzer (ADA) were applied used for the analysis of differentially differently expressed mRNAs. mRNA expression was verified by quantitative real-timer reverse transcriptiontase polymerase chain reaction (qRT-PCR). Immunohistochemistry were was used to show natural killer-tumor recognition (NKTR) expression in CRC. NKTR-knockdown CRC cells were constructed obtained by using short hairpin RNA (shRNA). Followed by CCK-8 assay, plate colony formation test, and transwell assay were used to evaluate the influence of NKTR on cel

SUBMITTER: Bai R 

PROVIDER: S-EPMC9467399 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature