De novo transcriptome analysis of Justicia adhatoda reveals candidate genes involved in major biosynthetic pathway.

ABSTRACT:

Background

Justicia adhatoda is an important medicinal plant traditionally used in the Indian system of medicine and the absence of molecular-level studies in this plant hinders its wide use, hence the study was aimed to analyse the genes involved in its various pathways.

Methods and results

The RNA isolated was subjected to Illumina sequencing. De novo assembly was performed using TRINITY software which produced 171,064 transcripts with 55,528 genes and N50 value of 2065 bp, followed by annotation of unigenes against NCBI, KEGG and Gene ontology databases resulted in 105,572 annotated unigenes and 40,288 non-annotated unigenes. A total of 5980 unigenes were mapped to 144 biochemical pathways, including the metabolism and biosynthesis pathways. The pathway analysis revealed

SUBMITTER: Padmanabhan D 

PROVIDER: S-EPMC9467423 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature