Mutation in Drosophila concentrative nucleoside transporter 1 alters spermatid maturation and mating behavior.

ABSTRACT: Concentrative nucleoside transporters (Cnts) are unidirectional carriers that mediate the energy-costly influx of nucleosides driven by the transmembrane sodium gradient. Cnts are transmembrane proteins that share a common structural organization and are found in all phyla. Although there have been studies on Cnts from a biochemical perspective, no deep research has examined their role at the organismal level. Here, we investigated the role of the Drosophila melanogaster cnt1 gene, which is specifically expressed in the testes. We used the CRISPR/Cas9 system to generate a mutation in the cnt1 gene. The cnt1 mutants exhibited defects in the duration of copulation and spermatid maturation, which significantly impaired male fertility. The most striking effect of the cn

SUBMITTER: Maaroufi HO 

PROVIDER: S-EPMC9467524 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature