Development and Characterization of Synthetic Norovirus RNA for Use in Molecular Detection Methods.

ABSTRACT:

Background

Reference materials are essential for the quality assurance of molecular detection methods. We developed and characterized synthetic norovirus GI and GII RNA reference materials.

Methods

Norovirus GI and GII RNA sequences including the ORF1-ORF2 junction region were designed based on 1,495 reported norovirus sequences and synthesized via plasmid preparation and in vitro transcription. The synthetic norovirus GI and GII RNAs were evaluated using six commercial norovirus detection kits used in Korea and subjected to homogeneity and stability analyses. A multicenter study involving five laboratories and using four commercial real-time PCR norovirus detection assays was conducted for synthetic norovirus RNA characterization and uncertainty measurements.

Resu

SUBMITTER: Cho EJ 

PROVIDER: S-EPMC9467847 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature