DNA-PAINT MINFLUX nanoscopy.

ABSTRACT: MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations, has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on-off switching process for single fluorophore separation, initial MINFLUX implementations have been limited to two color channels. Here we show that MINFLUX can be effectively combined with sequentially multiplexed DNA-based labeling (DNA-PAINT), expanding MINFLUX nanoscopy to multiple molecular targets. Our method is exemplified with three-color recordings of mitochondria in human cells.

SUBMITTER: Ostersehlt LM 

PROVIDER: S-EPMC9467913 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature