A high-performance genetically encoded fluorescent indicator for in vivo cAMP imaging.

ABSTRACT: cAMP is a key second messenger that regulates diverse cellular functions including neural plasticity. However, the spatiotemporal dynamics of intracellular cAMP in intact organisms are largely unknown due to low sensitivity and/or brightness of current genetically encoded fluorescent cAMP indicators. Here, we report the development of the new circularly permuted GFP (cpGFP)-based cAMP indicator G-Flamp1, which exhibits a large fluorescence increase (a maximum ΔF/F₀ of 1100% in HEK293T cells), decent brightness, appropriate affinity (a K_d of 2.17 μM) and fast response kinetics (an association and dissociation half-time of 0.20 and 0.087 s, respectively). Furthermore, the crystal structure of the cAMP-bound G-Flamp1 reveals one linker connecting the cAMP-binding domain

SUBMITTER: Wang L 

PROVIDER: S-EPMC9468011 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature