Cocaine's cerebrovascular vasoconstriction is associated with astrocytic Ca²⁺ increase in mice.

ABSTRACT: Human and animal studies have reported widespread reductions in cerebral blood flow associated with chronic cocaine exposures. However, the molecular and cellular mechanisms underlying cerebral blood flow reductions are not well understood. Here, by combining a multimodal imaging platform with a genetically encoded calcium indicator, we simultaneously measured the effects of acute cocaine on neuronal and astrocytic activity, tissue oxygenation, hemodynamics and vascular diameter changes in the mouse cerebral cortex. Our results showed that cocaine constricted blood vessels (measured by vessel diameter Φ changes), decreasing cerebral total blood volume (HbT) and temporally reducing tissue oxygenation. Cellular imaging showed that the mean astrocytic Ca²⁺ dependent fluorescence [F

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9468035 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature