Evaluation of fluorescence-based viability stains in cells dissociated from scleractinian coral Pocillopora damicornis.

ABSTRACT: The application of established cell viability assays such as the commonly used trypan blue staining method to coral cells is not straightforward due to different culture parameters and different cellular features specific to mammalian cells compared to marine invertebrates. Using Pocillopora damicornis as a model, we characterized the autofluorescence and tested different fluorescent dye pair combinations to identify alternative viability indicators. The cytotoxicity of different representative molecules, namely small organic molecules, proteins and nanoparticles (NP), was measured after 24 h of exposure using the fluorescent dye pair Hoechst 33342 and SYTOX orange. Our results show that this dye pair can be distinctly measured in the presence of fluorescent proteins plus chlorophyll. P. d

SUBMITTER: Roger LM 

PROVIDER: S-EPMC9468155 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature