TRIM7 modulates NCOA4-mediated ferritinophagy and ferroptosis in glioblastoma cells.

ABSTRACT:

Objective

Glioblastoma is one of the most common intracranial malignant tumors with an unfavorable prognosis, and iron metabolism as well as ferroptosis are implicated in the pathogenesis of glioblastoma. The present study aims to decipher the role and mechanisms of tripartite motif-containing protein 7 (TRIM7) in ferroptosis and glioblastoma progression.

Methods

Stable TRIM7-deficient or overexpressing human glioblastoma cells were generated with lentiviral vectors, and cell survival, lipid peroxidation and iron metabolism were evaluated. Immunoprecipitation, protein degradation and ubiquitination assays were performed to demonstrate the regulation of TRIM7 on its candidate proteins.

Results

TRIM7 expression was elevated in human glioblastoma cells and tissues. TRIM7

SUBMITTER: Li K 

PROVIDER: S-EPMC9468590 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature