Sibling rivalry among the ZBTB transcription factor family: homodimers versus heterodimers.

ABSTRACT: The BTB domain is an oligomerization domain found in over 300 proteins encoded in the human genome. In the family of BTB domain and zinc finger-containing (ZBTB) transcription factors, 49 members share the same protein architecture. The N-terminal BTB domain is structurally conserved among the family members and serves as the dimerization site, whereas the C-terminal zinc finger motifs mediate DNA binding. The available BTB domain structures from this family reveal a natural inclination for homodimerization. In this study, we investigated the potential for heterodimer formation in the cellular environment. We selected five BTB homodimers and four heterodimer structures. We performed cell-based binding assays with fluorescent protein-BTB domain fusions to assess dimer formation. We tested t

SUBMITTER: Piepoli S 

PROVIDER: S-EPMC9468604 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature