Mitochondrial metabolism determines the functional status of human sperm and correlates with semen parameters.

ABSTRACT: The diagnosis of male infertility is based essentially on the patient's medical history and a standard semen analysis. However, the latter rarely provides information on the causes of a possible infertility, emphasizing the need to extend the analysis of the sperm function. Mitochondrial function has been associated with sperm function and dysfunction, the latter primarily through the production of excessive amounts of reactive oxygen species (ROS). We hypothesized that analysis of sperm mitochondrial metabolism together with sperm ROS production could be an additional tool to improve routine semen analysis, after appropriate validations. To test our hypothesis, we performed several experiments using a non-routine method (high-resolution respirometry, HRR) to access mitochondrial function.

SUBMITTER: Irigoyen P 

PROVIDER: S-EPMC9468643 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature