Effect of Lenvatinib treatment on the cell cycle and microRNA profile in hepatocellular carcinoma cells.

ABSTRACT: Lenvatinib is a tyrosine kinase receptor inhibitor used to treat unresectable hepatocellular carcinoma (HCC). In this study, we investigated the antitumor effects of Lenvatinib treatment on HCC cell lines. Proliferation was examined in four HCC cell lines (HuH-7, Hep3B, Li-7, and PLC/PRF/5) using Cell Counting Kit-8 assays. Xenograft mouse models were used to assess the effects of Lenvatinib in vivo. Cell cycle, western blotting, and microRNA (miRNA) expression analyses were performed to identify the antitumor inhibitory potential of Lenvatinib on HCC cells. Lenvatinib treatment suppressed proliferation of HuH-7 and Hep3B, but not Li-7 and PLC/PRF/5 cells and induced G₀/G₁ cell cycle arrest and cyclin D1 downregulation in Lenvatinib-sensitive cells. Lenvatinib

SUBMITTER: Nakahara M 

PROVIDER: S-EPMC9468852 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature