Changes in patient peripheral blood cell microRNAs after total body irradiation during hematopoietic stem cell transplantation.

ABSTRACT:

Background

Ionizing radiation exposure is a great threat to human health. MicroRNAs (miRNAs) have been shown to play an important role in radiation-induced biological effects. Here, we investigated plasma miRNA expression changes and differentially expressed miRNAs in radiotherapy patients exposed to cobalt-60 (⁶⁰Co) gamma rays to provide an experimental basis for human plasma miRNAs as an estimation indicator for ionizing radiation injury.

Methods

Six patients with acute lymphoblastic leukemia (ALL) received continuous 5 gray (Gy) total body irradiation (TBI) twice. At 12 hours after irradiation, miRNA microarray was applied to screen for differentially expressed miRNAs, with some miRNAs confirmed by real-time polymerase chain reaction (RT-PCR) assay. Bioinformat

SUBMITTER: Li JJ 

PROVIDER: S-EPMC9469155 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature