An IGF-1R-mTORC1-SRPK2 signaling Axis contributes to FASN regulation in breast cancer.

ABSTRACT:

Background

Fatty acid synthase (FASN) expression is associated with a more aggressive breast cancer phenotype and is regulated downstream of receptor tyrosine kinase (RTK) signaling pathways. Recently, post transcriptional regulation of lipogenic transcripts have been demonstrated as being mediated downstream of serine-arginine rich protein kinase 2 (SRPK2), which acts to phosphorylate serine-arginine rich splicing factors (SRSFs), resulting in RNA binding and various RNA regulatory processes. Though post-transcriptional regulation of FASN has been studied previously, the upstream mediators of these pathways have not been elucidated.

Methods

Western blotting and RT-qPCR were utilized to demonstrate alterations in FASN and mRNA expression upon modulation of the IGF-1-mTORC1-S

SUBMITTER: McClellan B 

PROVIDER: S-EPMC9469522 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature