Siderophores Produced by the Fish Pathogen Flavobacterium columnare Strain MS-FC-4 Are Not Essential for Its Virulence.

ABSTRACT: Flavobacterium columnare causes columnaris disease in wild and aquaculture-reared freshwater fish. F. columnare virulence mechanisms are not well understood, and current methods to control columnaris disease are inadequate. Iron acquisition from the host is important for the pathogenicity and virulence of many bacterial pathogens. F. columnare iron acquisition has not been studied in detail. We identified genes predicted to function in siderophore production for ferric iron uptake. Genes predicted to encode the proteins needed for siderophore synthesis, export, uptake, and regulation were deleted from F. columnare strain MS-FC-4. The mutants were examined for defects in siderophore production, for growth defects in iron-limited conditions, and for virulence against zebrafish and rainbow tr

SUBMITTER: Conrad RA 

PROVIDER: S-EPMC9469716 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature