Spatiotemporal single-cell profiling reveals that invasive and tissue-resident memory donor CD8⁺ T cells drive gastrointestinal acute graft-versus-host disease.

ABSTRACT: Organ infiltration by donor T cells is critical to the development of acute graft-versus-host disease (aGVHD) in recipients after allogeneic hematopoietic stem cell transplant (allo-HCT). However, deconvoluting the transcriptional programs of newly recruited donor T cells from those of tissue-resident T cells in aGVHD target organs remains a challenge. Here, we combined the serial intravascular staining technique with single-cell RNA sequencing to dissect the tightly connected processes by which donor T cells initially infiltrate tissues and then establish a pathogenic tissue residency program in a rhesus macaque allo-HCT model that develops aGVHD. Our results enabled creation of a spatiotemporal map of the transcriptional programs controlling donor CD8⁺ T cell infiltration into

SUBMITTER: Tkachev V 

PROVIDER: S-EPMC9469805 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature