Sigma(54) Promoters control expression of genes encoding the hook and basal body complex in Rhodobacter sphaeroides.

ABSTRACT: Gene expression of the flagellar system is tightly controlled by external stimuli or intracellular signals. A general picture of this regulation has been obtained from studies of Salmonella enterica serovar Typhimurium. However, these regulatory mechanisms do not apply to all bacterial groups. In this study, we have investigated regulation of the flagellar genetic system in Rhodobacter sphaeroides. Deletion analysis, site-directed mutagenesis, and 5'-end mapping were conducted in order to identify the fliO promoter. Our results indicate that this promoter is recognized by the factor sigma(54). Additionally, 5'-end mapping of the flgB and fliK transcripts suggests that these mRNAs are also transcribed from sigma(54) promoters. Finally, we showed evidence that suggests that fliC transcriptio

SUBMITTER: Poggio S 

PROVIDER: S-EPMC94701 | biostudies-literature | 2000 Oct

REPOSITORIES: biostudies-literature