Monoclonal antibody-mediated immunosuppression enables long-term survival of transplanted human neural stem cells in mouse brain.

ABSTRACT:

Background

As the field of stem cell therapy advances, it is important to develop reliable methods to overcome host immune responses in animal models. This ensures survival of transplanted human stem cell grafts and enables predictive efficacy testing. Immunosuppressive drugs derived from clinical protocols are frequently used but are often inconsistent and associated with toxic side effects. Here, using a molecular imaging approach, we show that immunosuppression targeting costimulatory molecules CD4 and CD40L enables robust survival of human xenografts in mouse brain, as compared to conventional tacrolimus and mycophenolate mofetil.

Methods

Human neural stem cells were modified to express green fluorescent protein and firefly luciferase. Cells were implanted in the fimbria

SUBMITTER: McGinley LM 

PROVIDER: S-EPMC9471059 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature