MiR-4739/ITGA10/PI3K signaling regulates differentiation and apoptosis of osteoblast.

ABSTRACT:

Introduction

To probe the impacts and biological roles of miR-4739/ITGA10 on the proliferation, differentiation and apoptosis of osteoblasts.

Methods

Bioinformatics analysis was conducted to screen the key genes in osteoporosis. The upstream miRNAs of ITGA10 were predicted by TargetScan. KEGG pathway enrichment analysis was performed by DAVID database. The osteoblast proliferation and apoptosis were measured using CCK-8 and flow cytometry. The differentiation markers were measured by qRT-PCR and western blotting. The luciferase reporter assay was conducted to verify the binding of miR-4739 to ITGA10.

Results

ITGA10 was down-regulated in patients with osteoporosis and identified as the key gene in osteoporosis by the bioinformatics analysis. Then the prediction provide

SUBMITTER: Song Y 

PROVIDER: S-EPMC9471362 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature