Dataset Information

Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery.

ABSTRACT: The tea plant is a kind of ammonium-preferring crop, but the mechanism whereby ammonium (NH₄ ⁺) regulate its growth is not well understood. The current study focused on the effects of NH₄ ⁺ on tea plants. Transcriptomic analysis was performed to investigate the early- and late-stage NH₄ ⁺ deprivation and resupply in tea plants shoots. Through short- and long-term NH₄ ⁺ deficiency, the dynamic response to NH₄ ⁺ stress was investigated. The most significant effects of NH₄ ⁺ deficiency were found to be on photosynthesis and gene ontology (GO) enrichment varied with the length of NH₄ ⁺ deprivation. Enriched KEGG pathways were also different when NH₄ ⁺ was resupplied at different concentrations which may indicate reasons for tolerance of high NH₄ ⁺ concentration. Using weighted gene co-expression network analysis (WGCNA), modules related to significant tea components, tea polyphenols and free amino acids, were identified. Hence, NH₄ ⁺ could be regarded as a signaling molecule with the response of catechins shown to be higher than that of amino acids. The current work represents a comprehensive transcriptomic analysis of plant responses to NH₄ ⁺ and reveals many potential genes regulated by NH₄ ⁺ in tea plants. Such findings may lead to improvements in nitrogen efficiency of tea plants.

SUBMITTER: Wang Y

PROVIDER: S-EPMC9472221 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery.

Wang Yu Y Ouyang Jia-Xue JX Fan Dong-Mei DM Wang Shu-Mao SM Xuan Yi-Min YM Wang Xiao-Chang XC Zheng Xin-Qiang XQ

Frontiers in plant science 20220830

The tea plant is a kind of ammonium-preferring crop, but the mechanism whereby ammonium (NH4 +) regulate its growth is not well understood. The current study focused on the effects of NH4 + on tea plants. Transcriptomic analysis was performed to investigate the early- and late-stage NH4 + deprivation and resupply in tea plants shoots. Through short- and long-term NH4 + deficiency, the dynamic response to NH<s ...[more]

PMID: 36119592

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Project description:Background: Lysine crotonylation (Kcr), as a novel evolutionarily conserved type of PTM, is ubiquitous and essential in cell biology. However, its functions in tea plant, an important beverage crop, are largely unknown. Our study firstly attempted to describe Kcr proteins in tea leaves under NH4+ deficiency/resupply, and provided significant insights into exploring the physiological role of Kcr in plants for N utilization. Results: We performed the global analysis of crotonylome in tea plants under NH4+ deficiency/resupply using high-resolution LC-MS/MS coupled with highly sensitive immune-antibody. A total of 2288 kcr sites on 971 proteins were identified, of which contained in 15 types of Kcr motifs. Most of Kcr proteins were located in chloroplast and cytoplasm. 120 and 151 Kcr proteins were significantly changed at 3 hours and 3days of NH4+ resupply, respectively. Bioinformatics analysis showed that differentially expressed Kcr proteins participated in diverse biological processes such as photosynthesis, carbon fixation and amino acid metabolism, suggesting Kcr plays important roles in these processes. Interestingly, a large number of enzymes were crotonylated, and the activity and Kcr level of these enzymes changed significantly after NH4+ resupply, indicating a potential function of Kcr in the regulation of enzyme activities. Moreover, the protein-protein interaction analysis revealed that the diverse interactions of identified Kcr proteins mainly involved in photosynthesis, carbon fixation, amino acid metabolism and ribosome. Conclusions: The results suggested that lysine crotonylated proteins might play regulating roles in metabolic process in tea leaves under NH4+ deficiency/resupply. The critical regulatory roles mainly involved in diverse aspects of primary metabolic processes, especially in photosynthesis, carbon fixation and amino acid metabolism. The provided data may serve as important resources for exploring the physiological, biochemical, and genetic role of lysine crotonylation in tea plants.

Project description:Proper postharvest storage preserves horticultural products, including tea, until they can be processed. However, few studies have focused on the physiology of ripening and senescence during postharvest storage, which affects the flavor and quality of tea. In this study, physiological and biochemical indexes of the leaves of tea cultivar 'Yinghong 9' preserved at a low temperature and high relative humidity (15-18 °C and 85-95%, PTL) were compared to those of leaves stored at ambient conditions (24 ± 2 °C and relative humidity of 65% ± 5%, UTL). Water content, chromatism, chlorophyll fluorescence, and key metabolites (caffeine, theanine, and catechins) were analyzed over a period of 24 h, and volatilized compounds were determined after 24 h. In addition, the expression of key biosynthesis genes for catechin, caffeine, theanine, and terpene were quantified. The results showed that water content, chromatism, and chlorophyll fluorescence of preserved leaves were more similar to fresh tea leaves than unpreserved tea leaves. After 24 h, the content of aroma volatiles and caffeine significantly increased, while theanine decreased in both groups. Multiple catechin monomers showed distinct changes within 24 h, and EGCG was significantly higher in preserved tea. The expression levels of CsFAS and CsTSI were consistent with the content of farnesene and theanine, respectively, but TCS1 and TCS2 expression did not correlate with caffeine content. Principal component analysis considered results from multiple indexes and suggested that the freshness of PTL was superior to that of UTL. Taken together, preservation conditions in postharvest storage caused a series of physiological and metabolic variations of tea leaves, which were different from those of unpreserved tea leaves. Comprehensive evaluation showed that the preservation conditions used in this study were effective at maintaining the freshness of tea leaves for 2-6 h. This study illustrates the metabolic changes that occur in postharvest tea leaves, which will provide a foundation for improvements to postharvest practices for tea leaves.

Dataset Information

Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery.

Publications

Transcriptome analysis of tea (<i>Camellia sinensis</i>) leaves in response to ammonium starvation and recovery.

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