Reversing BCG-mediated autophagy inhibition and mycobacterial survival to improve vaccine efficacy.

ABSTRACT:

Background

Autophagy is an important mechanism for promoting Mycobacterium clearance from macrophages. Pathogenic and non-pathogenic mycobacterium can activate the mTOR pathway while simultaneously inducing autophagy. M. tuberculosis and M. bovis BCG inhibit autophagy and favor intracellular bacteria survival.

Results

We observed that pre-infection of live or heat-killed BCG could prevent autophagy induced by pharmacological activators or M. smegmatis, a strong autophagy-inducing mycobacterium. BCG-derived lipids are responsible for autophagy inhibition. However, post-infection with BCG could not stop the autophagy initiated by M. smegmatis, which increases further autophagy induction and mycobacteria clearance. Coinfection with BCG and heat killed M. smegmatis enhanced anti

SUBMITTER: Gonzalez-Orozco M 

PROVIDER: S-EPMC9472362 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature