Glucose consumption rate-dependent transcriptome profiling of Escherichia coli provides insight on performance as microbial factories.

ABSTRACT:

Background

The modification of glucose import capacity is an engineering strategy that has been shown to improve the characteristics of Escherichia coli as a microbial factory. A reduction in glucose import capacity can have a positive effect on production strain performance, however, this is not always the case. In this study, E. coli W3110 and a group of four isogenic derivative strains, harboring single or multiple deletions of genes encoding phosphoenolpyruvate:sugar phosphotransferase system (PTS)-dependent transporters as well as non-PTS transporters were characterized by determining their transcriptomic response to reduced glucose import capacity.

Results

These strains were grown in bioreactors with M9 mineral salts medium containing 20 g/L of glucose, where they disp

SUBMITTER: Fragoso-Jimenez JC 

PROVIDER: S-EPMC9472385 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature