Using single-worm RNA sequencing to study C. elegans responses to pathogen infection.

ABSTRACT:

Background

Caenorhabditis elegans is an excellent research model whose populations have been used in many studies to address various biological questions. Although worm-to-worm phenotypic variations in isogenic populations have been persistently observed, they are not well understood and are often ignored or averaged out in studies, masking the impacts of such variations on data collection and interpretation. Single-worm RNA sequencing that profiles the transcriptomes of individual animals has the power to examine differences between individuals in a worm population, but this approach has been understudied. The integrity of the starting RNA, the quality of the library and sequence data, as well as the transcriptome-profiling effectiveness of single-worm RNA-seq remain unclear. Ther

SUBMITTER: Wang AJ 

PROVIDER: S-EPMC9472404 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature