RBM45 is an m⁶A-binding protein that affects neuronal differentiation and the splicing of a subset of mRNAs.

ABSTRACT: N⁶-methyladenosine (m⁶A) is deposited co-transcriptionally on thousands of cellular mRNAs and plays important roles in mRNA processing and cellular function. m⁶A is particularly abundant within the brain and is critical for neurodevelopment. However, the mechanisms through which m⁶A contributes to brain development are incompletely understood. RBM45 acts as an m⁶A-binding protein that is highly expressed during neurodevelopment. We find that RBM45 binds to thousands of cellular RNAs, predominantly within intronic regions. Rbm45 depletion disrupts the constitutive splicing of a subset of target pre-mRNAs, leading to altered mRNA and protein levels through both m⁶A-dependent and m⁶A-independent mechanisms. Finally,

SUBMITTER: Choi SH 

PROVIDER: S-EPMC9472474 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature