Role of VP30 Phosphorylation in Ebola Virus Nucleocapsid Assembly and Transport.

ABSTRACT: Ebola virus (EBOV) VP30 regulates viral genome transcription and replication by switching its phosphorylation status. However, the importance of VP30 phosphorylation and dephosphorylation in other viral replication processes such as nucleocapsid and virion assembly is unclear. Interestingly, VP30 is predominantly dephosphorylated by cellular phosphatases in viral inclusions, while it is phosphorylated in the released virions. Thus, uncertainties regarding how VP30 phosphorylation in nucleocapsids is achieved and whether VP30 phosphorylation provides any advantages in later steps in viral replication have arisen. In the present study, to characterize the roles of VP30 phosphorylation in nucleocapsid formation, we used electron microscopic analyses and live cell imaging systems. We identifie

SUBMITTER: Takamatsu Y 

PROVIDER: S-EPMC9472631 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature