Intracellular ratiometric temperature sensing using fluorescent carbon dots.

ABSTRACT: Highly sensitive non-invasive temperature sensing is critical for studying fundamental biological processes and applications in medical diagnostics. Nanoscale-based thermometers are promising non-invasive probes for precise temperature sensing with subcellular resolution. However, many of these systems have limitations as they rely on fluorescence intensity changes, deconvolution of peaks, or the use of hybrid systems to measure thermal events. To address this, we developed a fluorescence-based ratiometric temperature sensing approach using carbon dots prepared via microwave synthesis. These dots possess dual fluorescence signatures in the blue and red regions of the spectrum. We observed a linear response as a function of temperature in the range of 5-60 °C with a thermal resolutio

SUBMITTER: Macairan JR 

PROVIDER: S-EPMC9473198 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature