Mitochondrial regulation of acute extrafollicular B-cell responses to COVID-19 severity.

ABSTRACT:

Background

Patients with COVID-19 display a broad spectrum of manifestations from asymptomatic to life-threatening disease with dysregulated immune responses. Mechanisms underlying the detrimental immune responses and disease severity remain elusive.

Methods

We investigated a total of 137 APs infected with SARS-CoV-2. Patients were divided into mild and severe patient groups based on their requirement of oxygen supplementation. All blood samples from APs were collected within three weeks after symptom onset. Freshly isolated PBMCs were investigated for B cell subsets, their homing potential, activation state, mitochondrial functionality and proliferative response. Plasma samples were tested for cytokine concentration, and titer of Nabs, RBD-, S1-, SSA/Ro- and dsDNA-specific

SUBMITTER: Cao T 

PROVIDER: S-EPMC9473490 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature