Monitoring and modulating a catalytic hybridization circuit for self-adaptive bioorthogonal DNA assembly.

ABSTRACT: Constructing artificial domino nanoarchitectures, especially dynamic DNA circuits associated with the actuation of biological functions inside live cells, represents a versatile and powerful strategy to regulate the behaviors and fate of various living entities. However, the stepwise operation of conventional DNA circuits always relies on freely diffusing reactants, which substantially slows down their operation rate and efficiency. Herein, a self-adaptive localized catalytic circuit (LCC) is developed to execute the self-sustained bioorthogonal assembly of DNA nanosponges within a crowded intracellular environment. The LCC-generated DNA scaffolds are utilized as versatile templates for realizing the proximity confinement of LCC reactants. Single-molecule-detecting fluorescence correlation

SUBMITTER: Gong X 

PROVIDER: S-EPMC9473505 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature