Distinct gene expression patterns for CD14++ and CD16++ monocytes in preeclampsia.

ABSTRACT: Preeclampsia (PE) is a serious gestational complication affecting the life of a mother and child. The immunophenotype and gene expression profile of isolated blood monocyte subpopulations of pregnant women with PE have not been studied before. In this work, we assessed changes in CD14++ and CD16++ monocyte subpopulations in PE and physiological pregnancy (n = 33). Immunophenotyping, immunomagnetic sorting of monocytes and analysis of the transcriptional profile of their genes were carried out. The percentage of classical monocytes was significantly lower, while the intermediate fraction of monocytes was significantly higher in late-onset PE compared to control. Transcriptome analysis of late-onset PE classical CD14++ monocytes revealed significant activation of inflammation mediated by che

SUBMITTER: Vishnyakova P 

PROVIDER: S-EPMC9474473 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature