A blind benchmark of analysis tools to infer kinetic rate constants from single-molecule FRET trajectories.

ABSTRACT: Single-molecule FRET (smFRET) is a versatile technique to study the dynamics and function of biomolecules since it makes nanoscale movements detectable as fluorescence signals. The powerful ability to infer quantitative kinetic information from smFRET data is, however, complicated by experimental limitations. Diverse analysis tools have been developed to overcome these hurdles but a systematic comparison is lacking. Here, we report the results of a blind benchmark study assessing eleven analysis tools used to infer kinetic rate constants from smFRET trajectories. We test them against simulated and experimental data containing the most prominent difficulties encountered in analyzing smFRET experiments: different noise levels, varied model complexity, non-equilibrium dynamics, and kinetic he

SUBMITTER: Gotz M 

PROVIDER: S-EPMC9474500 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature