Layered feedback control overcomes performance trade-off in synthetic biomolecular networks.

ABSTRACT: Layered feedback is an optimization strategy in feedback control designs widely used in engineering. Control theory suggests that layering multiple feedbacks could overcome the robustness-speed performance trade-off limit. In natural biological networks, genes are often regulated in layers to adapt to environmental perturbations. It is hypothesized layering architecture could also overcome the robustness-speed performance trade-off in genetic networks. In this work, we validate this hypothesis with a synthetic biomolecular network in living E. coli cells. We start with system dynamics analysis using models of various complexities to guide the design of a layered control architecture in living cells. Experimentally, we interrogate system dynamics under three groups of perturbations. We cons

SUBMITTER: Hu CY 

PROVIDER: S-EPMC9474519 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature