Mettl3-dependent m⁶A modification attenuates the brain stress response in Drosophila.

ABSTRACT: N⁶-methyladenosine (m⁶A), the most prevalent internal modification on eukaryotic mRNA, plays an essential role in various stress responses. The brain is uniquely vulnerable to cellular stress, thus defining how m⁶A sculpts the brain's susceptibility may provide insight to brain aging and disease-related stress. Here we investigate the impact of m⁶A mRNA methylation in the adult Drosophila brain with stress. We show that m⁶A is enriched in the adult brain and increases with heat stress. Through m⁶A-immunoprecipitation sequencing, we show 5'UTR Mettl3-dependent m⁶A is enriched in transcripts of neuronal processes and signaling pathways that increase upon stress. Mettl3 knockdown results in increased levels of m

SUBMITTER: Perlegos AE 

PROVIDER: S-EPMC9474545 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature