Skeletal Effects of Inducible ERα Deletion in Osteocytes in Adult Mice.

ABSTRACT: Estrogen is known to regulate bone metabolism in both women and men, but substantial gaps remain in our knowledge of estrogen and estrogen receptor alpha (ERα) regulation of adult bone metabolism. Studies using global ERα-knockout mice were confounded by high circulating sex-steroid levels, and osteocyte/osteoblast-specific ERα deletion may be confounded by ERα effects on growth versus the adult skeleton. Thus, we developed mice expressing the tamoxifen-inducible CreERT2 in osteocytes using the 8-kilobase (kb) Dmp1 promoter (Dmp1^CreERT2 ). These mice were crossed with ERα^fl//fl mice to create ERαΔOcy mice, permitting inducible osteocyte-specific ERα deletion in adulthood. After intermittent tamoxifen treatment of adult 4-month-old mice for 1 month, female, but not mal

SUBMITTER: Doolittle ML 

PROVIDER: S-EPMC9474695 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature