A Versatile Micromanipulation Apparatus for Biophysical Assays of the Cell Nucleus.

ABSTRACT:

Intro

Force measurements of the nucleus, the strongest organelle, have propelled the field of mechanobiology to understand the basic mechanical components of the nucleus and how these components properly support nuclear morphology and function. Micromanipulation force measurement provides separation of the relative roles of nuclear mechanical components chromatin and lamin A.

Methods

To provide access to this technique, we have developed a universal micromanipulation apparatus for inverted microscopes. We outline how to engineer and utilize this apparatus through dual micromanipulators, fashion and calibrate micropipettes, and flow systems to isolate a nucleus and provide force vs. extensions measurements. This force measurement approach provides the unique ability to measur

SUBMITTER: Currey ML 

PROVIDER: S-EPMC9474788 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature