Vitrification within a nanoliter volume: oocyte and embryo cryopreservation within a 3D photopolymerized device.

ABSTRACT:

Purpose

Vitrification permits long-term banking of oocytes and embryos. It is a technically challenging procedure requiring direct handling and movement of cells between potentially cytotoxic cryoprotectant solutions. Variation in adherence to timing, and ability to trace cells during the procedure, affects survival post-warming. We hypothesized that minimizing direct handling will simplify the procedure and improve traceability. To address this, we present a novel photopolymerized device that houses the sample during vitrification.

Methods

The fabricated device consisted of two components: the Pod and Garage. Single mouse oocytes or embryos were housed in a Pod, with multiple Pods docked into a Garage. The suitability of the device for cryogenic application was assessed by

SUBMITTER: Yagoub SH 

PROVIDER: S-EPMC9474789 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature