Selective single-bacteria extraction based on capture and release of microemulsion droplets.

ABSTRACT: Human host-associated microbial communities in body sites can reflect health status based on the population distribution and specific microbial properties in the heterogeneous community. Bacteria identification at the single-cell level provides a reliable biomarker and pathological information for clinical diagnosis. Nevertheless, biosamples obtained from some body sites cannot offer sufficient sample volume and number of target cells as required by most of the existing single-cell isolation methods such as flow cytometry. Herein we report a novel integrated microfluidic system, which consists of a microemulsion module for single-bacteria encapsulation and a sequential microdroplet capture and release module for selectively extracting only the single-bacteria encapsulated in microdroplets.

SUBMITTER: Li J 

PROVIDER: S-EPMC9474873 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature