Mitochondrial impairment and intracellular reactive oxygen species alter primary cilia morphology.

ABSTRACT: Primary cilia have recently emerged as cellular signaling organelles. Their homeostasis and function require a high amount of energy. However, how energy depletion and mitochondria impairment affect cilia have barely been addressed. We first studied the spatial relationship between a mitochondria subset in proximity to the cilium in vitro, finding similar mitochondrial activity measured as mitochondrial membrane potential compared with the cellular network. Next, using common primary cilia cell models and inhibitors of mitochondrial energy production, we found alterations in cilia number and/or length due to energy depletion and mitochondrial reactive oxygen species (ROS) overproduction. Finally, by using a mouse model of type 2 diabetes mellitus, we provided in vivo evidence that cilia mo

SUBMITTER: Moruzzi N 

PROVIDER: S-EPMC9475181 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature