Tumor suppressor effect of an antibody on xenotransplanted sarcomatoid mesothelioma cells.

ABSTRACT:

Background

As mesothelioma generally has an unfavorable prognosis, further advances are needed to improve the outcomes in patients with mesothelioma. In the present study, we generated and characterized a monoclonal antibody that could inhibit mesothelioma cell proliferation in a xenotransplantation mouse model.

Methods

We generated monoclonal antibodies by immunizing mice with cultured mesothelioma cells. These antibodies were then characterized by immunofluorescence staining, immunohistochemical staining, secondary antibody-drug conjugate assay, antibody inoculation in a xenotransplantation mesothelioma mouse model, and mass spectrometry followed by small interfering RNA (siRNA) analysis. 5' rapid amplification of complementary DNA ends followed by sequencing was performed

SUBMITTER: Hasegawa M 

PROVIDER: S-EPMC9475231 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature