Extracellular vesicles on demand (EVOD) chip for screening and quantification of cancer-associated extracellular vesicles.

ABSTRACT: While significant advancements have been made in cancer therapeutics and treatments, early disease detection and diagnosis remains critical to ensuring favorable outcomes for patients. To that end, we propose a microfluidic based approach to the sensitive detection of an intriguing cancer biomarker, extracellular vesicles (EVs). Our extracellular vesicles on demand (EVOD) chip utilizes a catalyst-free click chemistry to rapidly and specifically isolate EVs of interest. This specific isolation is followed by subsequent dithiothreitol release of the isolated EVs for downstream functional analysis. This joint isolation and release provide a powerful tool for the screening and quantification of EVs of interest. By incorporating antibodies against cancer associated surface proteins into the cli

SUBMITTER: Kang YT 

PROVIDER: S-EPMC9475444 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature