Generation and in vivo validation of an IL-12 fusion protein based on a novel anti-human FAP monoclonal antibody.

ABSTRACT:

Background

In this study, we describe the generation of a fully human monoclonal antibody (named '7NP2') targeting human fibroblast activation protein (FAP), an antigen expressed in the microenvironment of different types of solid neoplasms.

Methods

7NP2 was isolated from a synthetic antibody phage display library and was improved by one round of mutagenesis-based affinity maturation. The tumor recognition properties of the antibody were validated by immunofluorescence procedures performed on cancer biopsies from human patients. A fusion protein consisting of the 7NP2 antibody linked to interleukin (IL)-12 was generated and the anticancer activity of the murine surrogate product (named mIL12-7NP2) was evaluated in mouse models. Furthermore, the safety of the fully human prod

SUBMITTER: Nadal L 

PROVIDER: S-EPMC9476130 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature