Th2-Biased Transcriptional Profile Predicts HIV Envelope-Specific Polyfunctional CD4⁺ T Cells That Correlated with Reduced Risk of Infection in RV144 Trial.

ABSTRACT: Ag-specific T cells play a critical role in responding to viral infections. In the RV144 HIV vaccine clinical trial, a rare subset of HIV-specific polyfunctional CD4⁺ T cells correlated with reduced risk of HIV-1 infection. Polyfunctional T cells are a subset of Ag-specific T cells that are able to simultaneously produce multiple effector cytokines. Little is known about what differentiates polyfunctional T cells from other vaccine-elicited T cells in humans. Therefore, we developed a novel live-cell multiplexed cytokine capture assay to identify, isolate, and transcriptionally profile vaccine-specific polyfunctional CD4⁺ T cells. We applied these methods to samples from subjects who received the RV144 vaccine regimen, as part of the HVTN 097 clinical trial. We identi

SUBMITTER: Cohen KW 

PROVIDER: S-EPMC9476163 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature