Dataset Information

Simultaneous Detection of Ochratoxin A and Aflatoxins in Industrial and Traditional Red and isot Pepper Flakes along with Dietary Exposure Risk Assessment.

ABSTRACT: This study focused on the co-occurrence of aflatoxins (AFs) and ochratoxin A (OTA) in traditionally and industrially dried red pepper flakes (DRPFs) and isot pepper flakes (IPFs). Following the multitoxin immunoaffinity column (IAC) clean-up, high-performance liquid chromatography coupled with fluorescence detection (HPLC-FLD) was used to quantify AFs and OTA. The limit of detection (LOD) and limit of quantification (LOQ) values were 0.11 and 0.18 μg kg^-1 (AFB₁), 0.04 and 0.08 μg kg^-1 (AFB₂), 0.13 and 0.18 μg kg^-1 (AFG₁), 0.04 and 0.11 μg kg^-1 (AFG₂), and 0.10 and 0.21 μg kg^-1 (OTA), respectively. AFB_1, AFB₂, AFG₁, and OTA were found to be positive in 93, 74, 17, and 94% of all samples, respectively. The contamination levels in positive samples ranged from 0.23 to 38.69, 0.04 to 2.14, 0.13 to 0.88, and 0.18 to 52.19 μg kg^-1 for AFB₁, AFB₂, AFG₁, and OTA, respectively, while no AFG₂ was found above the detection limit (0.04 μg kg^-1). None of the industrial isot peppers exceeded the European Union limits, while the levels of AFB₁ (5 μg kg^-1), total AFs (10 μg kg^-1), and OTA (20 μg kg^-1) of the traditional peppers were above the limit by 30% (16/54), 26% (14/54), and 4% (2/54), respectively. Co-occurrence of AFB₁-AFB₂-OTA was the most frequent, accounting for 54% (29/54) of all samples. At the upper bound (UB), estimated average exposure to AFB₁, total AFs, and OTA was determined to be 0.175, 0.189, and 0.124 ng kg^-1 bw day^-1 in all samples, respectively. The margin of exposure (MoE) value of AFB₁ and total AFs was found to be 977 and 909, indicating high health concerns compared to OTA (MoE >10,000). AFB₁ and total AFs may result in 0.0058 and 0.0062 liver cancer cases/100,000 person/year at UB, respectively, and weekly OTA exposure was 0.868 ng kg^-1 bw, well below the provisional tolerable weekly intake, hence not of health concern. AFs exposure could endanger health, whereas OTA posed no toxicological concerns through dried red pepper consumption.

SUBMITTER: Oztekin S

PROVIDER: S-EPMC9476180 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Simultaneous Detection of Ochratoxin A and Aflatoxins in Industrial and Traditional Red and isot Pepper Flakes along with Dietary Exposure Risk Assessment.

Oztekin Sebahat S Karbancioglu-Guler Funda F

ACS omega 20220826 36

This study focused on the co-occurrence of aflatoxins (AFs) and ochratoxin A (OTA) in traditionally and industrially dried red pepper flakes (DRPFs) and isot pepper flakes (IPFs). Following the multitoxin immunoaffinity column (IAC) clean-up, high-performance liquid chromatography coupled with fluorescence detection (HPLC-FLD) was used to quantify AFs and OTA. The limit of detection (LOD) and limit of quantification (LOQ) values were 0.11 and 0.18 μg kg-1 (AFB1), 0.0 ...[more]

PMID: 36120009

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Project description:As the most seriously controlled mycotoxin produced by Aspergillus spp. and Penicillium spp., ochratoxin A (OTA) results in various toxicological effects and widely contaminates agro-products. Biological detoxification is the highest priority regarding OTA in food and feed industry, but currently available detoxification enzymes have relatively low effectiveness in terms of time and cost. Here we show a superefficient enzyme, ADH3, identified from Stenotrophomonas acidaminiphila that has a strong ability to transform OTA into nontoxic ochratoxin-α by acting as an amidohydrolase. Recombinant ADH3 (1.2 μg/mL) completely degrades 50 μg/L OTA within 90 s, while the other most efficient OTA hydrolases available take several hours. The kinetic constant showed that rADH3 (Kcat/Km) catalytic efficiency was 56.7 to 35,000 times higher than those of previous hydrolases rAfOTase, rOTase, and commercial carboxypeptidase A (CPA). Protein structure-based assay suggested that ADH3 has a preference for hydrophobic residues to form a larger hydrophobic area than other detoxifying enzymes at the cavity of the catalytic sites, and this structure allows OTA easier access to the catalytic sites. In addition, ADH3 shows considerable temperature adaptability to exert hydrolytic function at the temperature down to 0°C or up to 70°C. Collectively, we report a superefficient OTA detoxifying enzyme with promising potential for industrial applications. IMPORTANCE Ochratoxin A (OTA) can result in various toxicological effects and widely contaminates agro-products and feedstuffs. OTA detoxifications by microbial strains and bio-enzymes are significant to food safety. Although previous studies showed OTA could be transformed through several pathways, the ochratoxin-α pathway is recognized as the most effective one. However, the most currently available enzymes are not efficient enough. Here, a superefficient hydrolase, ADH3, which can completely transform 50 μg/L OTA into ochratoxin-α within 90 s was screened and characterized. The hydrolase ADH3 shows considerable temperature adaptability (0 to 70°C) to exert the hydrolytic function. Findings of this study supplied an efficient OTA detoxifying enzyme and predicted the superefficient degradation mechanism, laying a foundation for future industrial applications.

Project description:Tribological studies of the 2D nanoadditives such as MoS2 and graphene are mostly performed in base oils such as SN500, SN150, or paraffin. We have focused on their effect in lubrication properties of industrial oils (e.g., axle, transmission, and compressor oils) along with SN500 oil employing a four-ball tester. Two types of graphene powders (GpowA with fewer defects than GpowC), MoS2 powder, and their physical mixtures are chosen as nanoadditives. The tribology performance for 0.05 wt% of additives in various industrial oils is evaluated by monitoring the coefficient of friction (COF) during rubbing and wear scar diameter (WSD) of the steel balls after rubbing. Elemental analysis and electron microscopy have been performed on the wear surfaces for evidence of any tribofilm formation. GpowA favors antifriction for axle and transmission oils with 40% reduction in axle oil, whereas it improved antiwear properties in most of the oils. GpowC shows a COF decrement by 12% only for compressor oil, but contribute to wear reduction in all oils. The observed COF reduction is attributed to the compatibility of nonfunctionalized GpowA with nonpolar axle oil and functionalized GpowC with polar compressor oil. MoS2 shows a decrease in the COF and WSD in most industrial oils; the best being 60% COF and 7% WSD reduction in axle oil. For additives in oils that favor antiwear, flakes or particles are observed on the wear surface supported by the higher elemental contribution of the constituents from the wear region. The mixtures of GpowA or C with MoS2, however, does not seem to favor improvement in the COF or WSD in industrial oils. With assistance from oleylamine surfactants, the lubrication properties of most additives are improved, particularly for the mixtures with 12-15% COF reduction and 4-7% WSD reduction in compressor oil. The study indicates that a large sheet size of high-quality graphene aids antifriction and addition of surfactant molecules facilitates a co-operative effect between MoS2 and graphene for improved tribology.

Dataset Information

Simultaneous Detection of Ochratoxin A and Aflatoxins in Industrial and Traditional Red and isot Pepper Flakes along with Dietary Exposure Risk Assessment.

Publications

Simultaneous Detection of Ochratoxin A and Aflatoxins in Industrial and Traditional Red and <i>isot</i> Pepper Flakes along with Dietary Exposure Risk Assessment.

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