The P2Y₁₁ receptor of human M2 macrophages activates canonical and IL-1 receptor signaling to translate the extracellular danger signal ATP into anti-inflammatory and pro-angiogenic responses.

ABSTRACT: The cytoprotective ATP receptor P2Y₁₁ is upregulated during M2 macrophage differentiation and contributes to the anti-inflammatory properties of this macrophage subset. Here, we studied P2Y₁₁-induced reprogramming of human M2 macrophages at the level of mRNA and protein expression. Upregulation of IL-1 receptor (IL-1R) and its known downstream effectors VEGF, CCL20 and SOCS3 as well as downregulation of the ATP-degrading ecto-ATPase CD39 emerged as hallmarks of P2Y₁₁ activation. The anti-inflammatory signature of the P2Y₁₁ transcriptome was further characterized by the downregulation of P2RX7, toll-like receptors and inflammasome components. P2Y₁₁-induced IL-1R upregulation formed the basis for reinforced IL-1 responsiveness of activat

SUBMITTER: Klaver D 

PROVIDER: S-EPMC9476423 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature