6,6'-Bieckol induces apoptosis and suppresses TGF-β-induced epithelial-mesenchymal transition in non-small lung cancer cells.

ABSTRACT:

Objective

In this study, the aim was to investigate the inhibitory effect of 6,6'-bieckol on the migration and epithelial-mesenchymal transition (EMT) of non-small cell lung cancer (NSCLC) cells, and explore its potential molecular mechanisms.

Methods

Cell migration was measured using a CCK8, wound healing, and transwell migration assay. Apoptosis was determined using an Annexin V/propidium iodide staining. Western blotting and immunofluorescence were used to examine the expression level of apoptosis-related proteins and EMT marker proteins.

Results

The results showed that 6,6'-bieckol inhibited migration and induced apoptosis of NSCLC cells. Furthermore, 6,6'-bieckol had significantly up-regulated the E-cadherin and down-regulated Snail1 and Twist1 transcriptional le

SUBMITTER: Li Y 

PROVIDER: S-EPMC9476679 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature